Influence of Sugar, Ammonium Nitrate and Plant Growth Regulators on in vitro Flowering of Celosia argentea var. cristata
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Keywords

 Ammonium nitrate, inflorescence, morphogenesis, plant growth regulators, sucrose.

How to Cite

Krupa-Ma kiewicz Marcelina, & Mg osiek Oktawia. (2016). Influence of Sugar, Ammonium Nitrate and Plant Growth Regulators on in vitro Flowering of Celosia argentea var. cristata. Journal of Basic & Applied Sciences, 12, 135–139. https://doi.org/10.6000/1927-5129.2016.12.20

Abstract

In vitro flowering is considered to be a complex process regulated by a vast of environmental and genetic factors. The present study describes the influence of sugar, ammonium nitrate and plant growth regulators on in vitroflowering from nodal explants of Celosia argentea var. cristata cultured for 6 weeks on basal MS medium under aseptic and light conditions. The addition to MS medium different concentrations of sucrose or ammonium nitrate did not influence on morphogenesis of Celosia plants. Among the plant growth regulators tested, supplementation with 1.0 mg/l KIN induced maximum number of new shoots (2.52). Flowering occurred on 100% of in vitro shoots cultured on modify MS medium supplemented with 16.50 and 8.25 g/l of NH4NO3 after 40 days.

https://doi.org/10.6000/1927-5129.2016.12.20
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References

Taha RM, Wafa SN. Plant regeneration and cellular behavior studies in Celosia cristata grown in vivo and in vitro. The Scientific Word Journal 2012. http://dx.doi.org/10.1100/2012/359413

Soltan ME, Sirry SM. Usefulness of some plant flowers as natural acid-base indicators. J of the Chinese Chemical Society 2002; 49: 63-68. http://dx.doi.org/10.1002/jccs.200200011

Bodhipadma K, Noichinda S, Yadbuntung I, Buaciam W. Comparison of in vitro and in vivo inflorescence of common cockscomb (Celosia argentea var. cristata). Sci Asia 2010; 36: 68-71. http://dx.doi.org/10.2306/scienceasia1513-1874.2010.36.068

Bakar DA, Ahmed BA, Taha RM. In vitro callus induction and plant regeneration of Celosia argentea – an important medicinal plant. Braz Arch Biol Technol 2014; 57: 860-866. http://dx.doi.org/10.1590/S1516-8913201402611

Ayodele JT, Olajide OS. Proximate and amino acid composition of Celosia argentea leaves. Niger. J. Basic Appl Sci 2011; 19: 162-165.

Saritha KV, Naidu CV. In vitro flowering of Withania somnifera Dunal. – An important antitumor medicinal plant. Plant Sci 2007; 172: 847-851. http://dx.doi.org/10.1016/j.plantsci.2006.12.016

Taylor NJ, van Staden J. Towards an understanding of the manipulation of in vitro flowering. In: Floriculture, ornamental and plant biotechnology: advances and topical issues. Da Silva J.A.T. (ed.), Global Science Books, London 2006; 2: 1-22.

Nadgauda RS, John CK, Parasharami VA, Joshi MS, Mascarenhas AF. A comparison of in vitro with in vivo flowering in bamboo: Bambusa arundinacea. Plant Cell, Tiss Organ Cult 1997; 48: 181-188. http://dx.doi.org/10.1023/A:1005800700024

Zhang Z, Leung DWM. Factors influencing the growth of micropropagated shoots and in vitro flowering of gentian. Plant Growth Reg 2002; 36: 245-251. http://dx.doi.org/10.1023/A:1016588302549

Murashige T, Skoog F. A revised medium, for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 1962; 15: 473-479. http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x

Daud N, Taha RM, Norr NNM, Alimon H. Provision of low cost media options for in vitro culture of Celosia sp. African J of Biotech 2011; 10: 18349-18355.

Yaacob JS, Saleh A, Elias H, Abdullah S, Mahmad N, Mohamed N. In vitro regeneration and acclimatization protocols of selected ornamental plants (Agapanthus praecox, Jucticia betonica and Celosia cristata). Sains Malaysiana 2014; 43: 715-722.

Franklin G, Pius PK, Ignacimuthu S. Factors affecting in vitro flowering and fruiting of green pea (Pisum sativum L.). Euphytica 2000; 115: 65-74. http://dx.doi.org/10.1023/A:1003982900117

Vu NH, Anh PH, Nhut DT. The role of sucrose and different cytokinins in the in vitro floral morphogenesis of rose (hybrid tea) cv. ‘First Prize’. Plant Cell, Tiss Organ Cult 2006 ; 87: 315-320. http://dx.doi.org/10.1007/s11240-006-9089-z

Parredy DR, Greyson RI. In vitro culture of immature tassels of an inbred field variety of Zea mays cv. Oh43. Plant Cell, Tiss Organ Cult 1985; 5: 119-128. http://dx.doi.org/10.1007/BF00040308

Zhang T. Studies on in vitro flowering and fruiting of Perilla frutescens. Agricultural Sci in China 2007; 6: 33-37. http://dx.doi.org/10.1016/S1671-2927(07)60014-5

Ignacimuthu S, Franklin G, Melchias G. Multiple shoot formation and in vitro fruiting of Vigna mungo L. Hepper. Curr Sci 1997; 73: 733-735.

Wang GY, Yuan MF, Hong Y. In vitro flower induction in roses. In vitro Cell Dev Biol – Plant 2002; 38: 513-518. http://dx.doi.org/10.1079/IVP2002340

Tee CS, Maziah M, Tan CS. Induction of in vitro flowering in the orchid Dendrobium Sonia17. Biol Plant 2008; 52: 723-726. http://dx.doi.org/10.1007/s10535-008-0139-8

Huang X, Yang B, Hu C, Yao J. In vitro induction of inflorescence in Dioscorea zingiberensis. Plant Cell, Tiss Org Cult 2009; 99: 209-215. http://dx.doi.org/10.1007/s11240-009-9595-x

Yamada J, Nakata K, Amaki W, Higuchi H. In vitro flowering in Celosia argentea var. cristata. Bull NODAI Res Inst 1997; 8: 22-40.

Bodhipadma K, Noichinda S, Padyencheun W, Khunthacharoen T, Chikhunthod U, Leung DWM. Influence of preculture and types of explants on shoot growth and in vitro flowering of feathered amaranth (Celosia argentea var. plumose). Plant Cell Tiss, Organ Cult 2011; 105: 465-469. http://dx.doi.org/10.1007/s11240-010-9883-5

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