Keywords
How to Cite
Abstract
Roses (Rosa indica L.) is one of the greatest significant flower collection in the biosphere and have commercial value in decorative, therapeutic and cosmetic trade. Speedy development and construction of disease free plants through in vitro technique have played a dynamic part in broadcast of salable rose cultivars. The five diverse meditations of growth controller such as (MS + 2.00 mg L-1 ), (IBA + 2.00 mg L-1 ), (AA + 30 gL-1 sucrose), (MS + 2.00 mg L-1), (BAP + 3.00 mg L-1 ), (NAA +30 mg L-1 sucrose), (MS + 2.00 mg L-1), (BAP + 3.00 mg L-1 ), ( IAA + 30 mg L-1 sucrose), (MS + 4 mg L-1), (BAP + 2.00 mg L-1), (( IAA + 30 mg L-1 sucrose) and (MS + 3.00 mg L-1 ), (BAP + 3.00 mg L-1), (IAA + 30 mg L-1 sucrose for shoot induction. After establishment of plantlets, stem was cast off as explants foundation for the initiation of callus. It is concluded that the two different concentrations 2.00 and 4.00 mg L-1 of 2 to 4 D were better worked out for callus induction.
References
Hameed N, Shabbir A, Ali A, Bajwa R. In vitro micropropagation of disease free rose (Rosaindica L.) Mycopath 2006; 4(2): 35-38.
Janarthanam B, Seshadri S. Plantlet regeneration from leaf derived callus of Vanilla planifolia Andr. In Vitro Cell Dev Biol Plant 2008; 44: 84-89. https://doi.org/10.1007/s11627-008-9123-4
Kumar S, Kanwar JK. Plant regeneration from cell suspension in Gerbera jamesonii Bolus. J Fru Orn Plant Res 2007; 15: 157-166.
Schneider F. Effect of different cultural conditions on micropropagation of rose (Rosa sp. L.) and globe artichoke (Cynarascolymus L.). PhD Thesis. Technische Universität MünchenWissen schaftszentrum Weihen Stephan für Ernährung, Landnutzung and Umwelt Department für Pflan zenwissen schaften Lehrstuhl für Zierp flanzenbau 2005.
Nosheen H, Shabbir A, Ali A, Bajwa R. In vitro micropropagation of disease free rose (Rosa indicaL.). Mycopath 2006; 4(2): 35-38.
Ferdous MH, Masum Billah AA, Mehraj H, Taufique T, Jamal Uddin AFS. BAP and IBA pulsing for in vitro multiplication of banana cultivars through shoot-tip culture. Journal of Bioscience and Agriculture Research 2015; 03(02): 87-95.
Gomez KA, Gomez AA. Statistical Procedure for Agricultural Research, (2 eds.). Wiley, New York, USA 1984; p. 680.
Tisserat B, Jones D. Clonal Propagation of Orchids. In: Hall RD (ed) Methods in Molecular Biology, Vol 111 Plant Cell Culture Protocols, Humana Press Inc, NJ 1999.
Rossi-Hassani BD, Zryad JP. In vitro culture and plant regeneration of large flowered purslane. Plant Cell Tiss Org Cult 1995; 41: 281-283. https://doi.org/10.1007/BF00045093
Tokuhara K, Mii M. Micropropagation of Phalaenopsis and Doritaenopsis by shoot tips of flower stalk buds. Plant Cell Rep 1993; 13: 7-11. https://doi.org/10.1007/BF00232306
Murashige T, Skoog F. A revised medium for rapid growth and bioassay with tissue culture. Physiol Plant 1962; 15: 473-476. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Analytical Software. Statistix 8.1 User’s Manual, Tallahassee, FL 2005.
Roy J, Banerjee N. Induction of callus and plant regeneration from shoot-tip explants of Dendrobiumfimbriatum Lindl var. oculatum Hk. F. Scientia Hortic 2003; 97: 333-340. https://doi.org/10.1016/S0304-4238(02)00156-5
Kumar S, Kanwar JK. Plant regeneration from cell suspension in Gerbera jamesonii Bolus. J Fru Orn Plant Res 2007; 15: 157-166.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright (c) 2018 Journal of Basic & Applied Sciences