Abstract
Phytophthora cinnamomi is an Oomycete considered one of the most widespread and destructive pathogens on the planet. Its geographic presence is cosmopolitan and the range of hosts is regarded as one of the largest. This pathogen causes enormous economic damages to important cultures worldwide; this has helped to attract the attention of the scientific community. It is the causal agent of chestnut ""ink"" disease (Castanea sativa Miller).
The chestnut tree has different applications, besides chestnut and wood, it plays an important role in soil ecology and biodiversity. Therefore it is important to know the molecular mechanisms (genes and proteins) that are the basis of the Castanea sativa infestation process by Phytophthora cinnamomi and the nature of the pathogen-host interaction in order to establish strategies to combat and control this parasite.
With bioinformatics tools, we were able to identify and characterize the Avr3a gene in genomic sequences of P. cinnamomi deposited in the Databases. This gene encodes a recognized 209 aminoacids, protein in the host cytoplasm where it triggers cell death.
References
Gomes-Laranjo J, Peixoto F, Ferreira-Cardoso J. Castanheiros, Técnicas e Práticas 1ª ed.; Pulido Consulting – Industria Criativa & Universidade de Trás-os-Montes e Alto Douro 2009.
Silva J. Árvores e Florestas de Portugal: Do castanheiro ao teixo. As outras espécies florestais; Lisboa: Publico, Comunicação Social, S.A. 2007; Vol. 5.
Costa R, Bragança H, Machado H. Os últimos 75 anos de investigação param o melhoramento do castanheiro em Portugal. Agrorrural - Contributos Científicos 2011; 915-926
Crandall S. The distribution and significance of the chestnut root rot Phytophthoras, P. cinnamomi and P. cambivora. Plant Dis Rep 1950; 34(6): 194-196.
Elorrieta J. El castaño en España. IFIE. Madrid, p333. Cit. by Gomes-Laranjo J, Peixoto F, Ferreira-Cardoso J. Castanheiros, Técnicas e Práticas. 1ª ed. (1949); Pulido Consulting – Industria Criativa & Universidade de Trás-os-Montes e Alto Douro 2009.
Fernandes C. A luta contra a “doença da tinta” dos castanheiros no Norte de Portugal. Separata das publicações da Direção Geral dos Serviços Florestais e Aquícolas 1953; Vol. XX: Tomo: 153-158.
Serrazina S. Isolamento e caracterização de genes de resistência à doença da tinta em Castanea sativa Mill. Universidade de Lisboa, Faculdade de Ciências 2004; Doctoral tesis.
Gouveia M. Doença da Tinta do Castanheiro. Avaliação da resistência à Phytophthora cinnamomi Rands. Universidade Técnica de Lisboa – Instituto Superior de Agronomia, Lisboa 1993; Master tesis.
Abreu C, Carvalho L, Gaspar M, Gomes A, Colaço J, Cardoso A. Assessment of resistance to chestnut ink disease. Acta Horticulturae 1999; 494: 363-367. https://doi.org/10.17660/ActaHortic.1999.494.55
Choupina A, Dominguez A, López M, Martins I. Isolation and Sequencing of Actin1, Actin2 and Tubulin1 Genes Involved in Cytoskeleton Formation in Phytophthora cinnamomi. Plant Pathology & Microbiology 2013; 4: 8.
Gouveia M. Um método eficiente para a detecção de Phytophthora cinnamomi associada com a Doença da Tinta do Castanheiro na rizosfera de Castanheiro (Castanea sativa Mill.). Revista de Ciencias Agrárias 2009; 32(1): 130-138.
Hwang S, Ko W. Biology of Chlamydospores, Sporangia, and Zoospores of Phytophthora cinnamomi in Soil. Phytopathology 1978; 68: 726-731. https://doi.org/10.1094/Phyto-68-726
Erwin D, Ribeiro O. Phytophthora diseases worldwide. St. Paul, Minnesota, USA: APS Press 1996.
Hawksworth D, Kirk P, Sutton B, Pegler D, Ainsworth & Bisby’s. Dictionary of the Fungi. Wallingford: Oxon CAB International 1995; 8th Ed., ISBN: 0 851 98 885 7.
Gouveia M. Métodos moleculares na identificação, caracterização e detecção de Phytophthora cambivora (Petri) Buisman e Phytophthora cinnamomi Rands associadas com a doença da tinta do castanheiro 2004; UTAD. Vila Real. Doctoral tesis.
Judelson H, Blanco F. The spores of Phytophthora: weapons of the plant destroyer. Nature Reviews Microbiology 2005; 3(1): 47-58. https://doi.org/10.1038/nrmicro1064
Shearer B, Tippett Jarrah. Dieback: The Dynamics and Management of Phytophthora cinnamomi in the Jarrah (Eucalytus marginata) Forest of South-western Australia. Research Bulletin N.º 3, Department of Conservation and Land Management Como Western Australia 1989.
Kimati H, Amorim L, Bergamim Filho A, Camargo L, Rezende J. Manual de Fitopatologia, Doenças das Plantas Cultivadas; Editora Agronômica Ceres Ltda, São Paulo 1997; Vol. 2.
Hosseini S. Expression patterns of pathogenicity genes during Phytophthora pisi infection of pea roots. Independent Project in biology 2010; Master’s thesis, 30 hp, EX0564.
Hogenhout S, Van der Hoorn R, Terauchi R, Kamoun S. Emerging conceptsin effector biology of plant-associated organisms. Molecular Plant- Microbe Interactions 2009; 22: 115-122. https://doi.org/10.1094/MPMI-22-2-0115
Kamoun S. A catalogue of the effector secretome of plant pathogenic oomycetes. Annual Review of Phytopathology 2006; 44: 41-60. https://doi.org/10.1146/annurev.phyto.44.070505.143436
Schornack S, Huitema E, Cano L, Bozkurt T, Oliva R, Van Damme M, Schwizer S, Raffaele S, Chaparro-Garcia A, Farrer R, Segretin M, Bos J, Haas B, Zody M, Nusbaum C, Win J, Thines M, Kamoun S. Ten things to knowabout oomycete effectors. Molecular Plant Pathology 2009; 10: 795-803. https://doi.org/10.1111/j.1364-3703.2009.00593.x
Hardham A. Pathogen profile: Phytothora cinnamomi. Molecular Plant Pathogy 2005; 6(6): 589-604. https://doi.org/10.1111/j.1364-3703.2005.00308.x
Duclos J, Fauconnier A, Coelho A, Bollen A, Cravator A, Godfroid E. Identification of an Elicitin Gene Cluster in Phytophthora cinnamomi. Dna Sequence: The journal of DNA sequencing and mapping 1998; 9(4): 231-237. https://doi.org/10.3109/10425179809105210
Kamoun S. Molecular Genetics of Pathogenic Oomycetes. Eukaryotic cell 2003; 10: 2(2): 191-199. https://doi.org/10.1128/EC.2.2.191-199.2003
Montesano M, Brader G, Palva T. Pathogen derived elicitors: searching for receptors in plants. Molecular Plant Pathology 2003; 4(1) 73-79. https://doi.org/10.1046/j.1364-3703.2003.00150.x
Zentmyer G. Phytophthora cinnamomi and the diseases it causes. Monograph, American Phytopathological Society 1980; N.º 10, pp. 96, pp. ref. 600. ISBN: 0890540306.
Moris B, Gow N. Mechanism of electrotaxis of zoospores of phytopathogenic fungi. Phytopathology 1993; 83: 877-882. https://doi.org/10.1094/Phyto-83-877
Stamps D, Waterhouse G, Newhook F, Hall G. Revised tabular key to the species of Phytophthora. Mycological Paper 162. Commonwealth Mycological Institute, Kew, Surrey, UK 1990.
Caetano P. Envolvimento de Phytophthora cinnamomi no declíneo de Quercus suber e Q. Rotundifolia: Estudo da influência de factores bióticos e abióticos na progressão da doença. Possibilidades de controlo químico do declíneo. Universidade do Algarve – Faculdade de Engenharia de Recursos Naturais 2007; Doctoral tesis.
Cahill D, Bennett I, McComb J. Mechanisms of resistance to Phytophthora cinnamomi in clonal, micropropagated Eucalyptus marginata. Plant Pathol 1993; 42(6): 865-872. https://doi.org/10.1111/j.1365-3059.1993.tb02672.x
Martins, M. Caracterização do gene gip de Phytophthora cinnamomi Rands associado à doença da Tinta do castanheiro e pesquisa de novos fitofármacos no controlo da doença. Instituto Politécnico de Bragança – Escola Superior Agrária 2010; Master tesis.
Martins M. Caracterização do gene gip de Phytophthora cinnamomi Rands associado à doença da Tinta do castanheiro e pesquisa de novos fitofármacos no controlo da doença. Instituto Politécnico de Bragança – Escola Superior Agrária. Tese de Mestrado 2010.
Götesson A, Marshall J, Hardham A. Characterization and evolutionary analysis of a large polygalacturonase gene family in the Oomycete plant pathogen Phytophthora cinnamomi. Molecular Plant-Microbe Interactions 2002; 15(9): 907-21. https://doi.org/10.1094/MPMI.2002.15.9.907
Choupina A, Cravador A, Carvalho M, Vaz M, Belo H, Martins F, Martins I. Cloning, characterization and in vitro and in planta expression of a glucanase inhibitor protein (GIP) of Phytophthora cinnamomi. Mol Biol Rep 2014; 41: 2453-2462. https://doi.org/10.1007/s11033-014-3101-1
Reitmann A, Berger D, van den Berg N. Putative pathogenicity genes of Phytophthora cinnamomi identified via RNA-Seq analysis of pre-infection structures. European Journal of Plant Pathology 2017; 147(1): 211-228. https://doi.org/10.1007/s10658-016-0993-8
Armstrong M, Whisson S, Pritchard L, Bos J, Venter E, Avrova A, Rehmany A, Bohme U, Brooks K, Cherevach I, Hamlin N, White B, Fraser A, Lord A, Quail M, Churcher C, Hall N, Berriman M, Huang S, Kamoun S, Beynon J, Birch P. An ancestral oomycete locus contains late blight avirulence gene Avr3a, encoding a protein that is recognised in the host cytoplasm. Proceedings of the National Academy of Sciences of the United States of America (PNAS) 2005; 102: 7766-7771. https://doi.org/10.1073/pnas.0500113102
Bos J, Armstrong M, Gilroy E. Phytophthora infestans effector AVR3a is essential for virulence and manipulates plant immunity by stabilizing host E3 ligase CMPG1. PNAS 2010; 107(21): 9909-9914. https://doi.org/10.1073/pnas.0914408107
Rapoport T. Transport of proteins across the endoplasmic reticulum membrane. Science 1992; 258: 931-936. https://doi.org/10.1126/science.1332192
Fry W, Goodwin S. Resurgence of the Irish potato famine fungus. Bioscience 1997; 47: 363-371. https://doi.org/10.2307/1313151
Tinoco M, Dias B, Dall`Astta R, Pamphile J, Aragão F. In vivo transspecific gene silencing in fungal cells by in plant expression of a double-stranded RNA. BMC Biology 2010; 8: 27. https://doi.org/10.1186/1741-7007-8-27
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright (c) 2020 Iuliia Branco , Altino Choupina