Abstract
The plant traditional medical application indicates great antioxidant and antimicrobial properties. The present study investigated the Biochemical and antimicrobial and antifungal activity of Periploca laevigata and Thymus algeriensis leaves extracts. The biochemical (total phenolic content, total flavonoids, and condensed tannins) and biological activity (antioxidant potentials, and antimicrobial activities) of leaf extractsof Periploca laevigata and Thymus algeriensis were assessed. The results showed that the leaves of the Thymus algeriensis extract has the highest levels of polyphenols, flavonoids, and tannins 120 ± 2.02 mg GAE/g DR, 46 ± 2.5 mg RE/g DR, and 22± 2mg CE/g DR, respectively. The GC-MS analyses showed that the plants haveinteresting volatile compounds. Therefore, leaves of Thymus algeriensis extract possess high anti-oxidant activities (total antioxidant capacity, 2,2-diphenyl-1-picrylhydrazyl and ferric reducing antioxidant power scavenging activities). In addition, this extract exhibited high antifungal and antimicrobial activities, especially toward Gram-positive bacteria. This results highlights ‘importance as dietary sources for natural antioxidants and antimicrobial can be used in traditional medicine and the pharmaceutical industry.
References
Hooker WJ. Editor Compendium of Potato Diseases. American Phytopathological Society Press. St. Paul, Minnesota 1986.
Hardham AR. The cell biology behind Phytophthora pathogenicity. Australas. Plant Pathol 2001; 30: 91-98. https://doi.org/10.1071/AP01006
Lacey J. The infectivity of soils containing Phytophthora infestans. Annals of applied Biology 1965; 56: 363-380. https://doi.org/10.1111/j.1744-7348.1965.tb01255.x
Greuter W, Burdet HM, Long G. Med-checklist: A critical inventory of vascular plants of the circum-Mediterranean countries. Conservatoire Jardin Botanique Ville de Genève 1986; 3: 395.
Flohe L, Gunzler WA. Assays of glutathione peroxidase. Methods Enzymology 1984; 105: 114-121. https://doi.org/10.1016/S0076-6879(84)05015-1
Pottier-Alapetite G. Flore de la Tunisie: Angiospermes Dicotylédones. Dialypétales 1979; 1: 654.
Athmouni K, Belhaj D, Mkadmini Hammi K, El Feki A, Ayadi H. Phenolic compounds analysis, antioxidant, and hepatoprotective effects of Periploca angustifolia extract on cadmium-induced oxidative damage in HepG2 cell line and rats. Archives of Physiology and Biochemistry 2017; 20: 1-14. https://doi.org/10.1080/13813455.2017.1395890
Belay WY, Gurmu AE, Wubneh ZB. Antimalarial Activity of Stem Bark of Periploca linearifolia during early and established plasmodium infection in mice. Evidence-Based Complementary and Alternative Medicine 2018; 7. https://doi.org/10.1155/2018/4169397
Iqbal J, Zaib S, Farooq U, Khan A, Bibi I, Suleman S. Antioxidant, antimicrobial, and free radical scavenging potential of aerial parts of Periploca aphylla and Ricinus communis. ISRN Pharmacology 2012; 6. https://doi.org/10.5402/2012/563267
Benkiniouar R, Rhouati S, Touil A, Seguin E, Chosson E. Flavonoids from Thymus algeriensis. Chemistry of Natural Compounds 2007; 43: 321-322. https://doi.org/10.1007/s10600-007-0118-x
Megdiche-ksouri W, Saada M, Soumay B, Snoussi M, Zaouali Y, Ksouri R. Potential use of wild Thymus algeriensis and Thymus capitatus as source of antioxidant and antimicrobial agents. Journal of New Sciences Agriculture and Biotechnology 2015; 23: 1046-1056.
Guesmi F, Ben Hadj Ahmed S, Landoulsi A. Investigation of extracts from Tunisian ethnomedicinal plants as antioxidants, cytotoxins, and antimicrobials. Biomedical and Environmental Sciences 2017; 30: 811-824.
Ziani BEC, Heleno SA, Bachari K, Dias MI, Alves MJ, Barros L, Ferreira ICFR. Phenolic compounds characterization by LC-DAD- ESI / MSn and bioactive properties of Thymus algeriensis Boiss. & Reut. and Ephedra alata Decne. Food Research International 2019; 116: 312-319. https://doi.org/10.1016/j.foodres.2018.08.041
Gadhoumi Hamza, Ben-Halima Emna, Walid Yeddes, Zohra Dhouafli, Moufida Tounsi S, Hayouni El Akrem. Chemical composition, antimicrobial and antioxidant activities data of three plants fromTunisia region: Erodium glaucophyllum, Erodium hirtum and Erodium guttatum. Data in Brief 2018; 19: 2352-2355. https://doi.org/10.1016/j.dib.2018.07.005
Folin O, Ciocalteu V. Tyrosine and tryptophan determination in proteins. J Biol Chem 1927; 27: 627-650.
Dewanto V, Wu X, Adom KK, Liu RH. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. Journal of Agricultural and Food Chemistry 2002; 50: 3010-3014. https://doi.org/10.1021/jf0115589
Broadhurst RB, Jones WT. Analysis of condensed tannins using acidified vanillin. J Sci Food Agric 1978; 29: 788-794. https://doi.org/10.1002/jsfa.2740290908
Heimler D, Vignolini P, Giulia Dini M, Francesco Vincieri F, Rmani A Antiradical activity and polyphenol composition of local Brassicaceae edible varieties. Food Chem 2006; 99: 464-469. https://doi.org/10.1016/j.foodchem.2005.07.057
Tian H, Shen Y, Yu H, He Y, Chen C. Effects of 4 probiotic strains in coculture with traditional starters on the flavor profile of yogurt. Journal of Food Science 2017; 82: 1693-1701. https://doi.org/10.1111/1750-3841.13779
Tlili N, Elfalleh W, Hannachi H, Yahia Y, Khaldi A, Ferchichi A. Screening of natural antioxidants from selected medicinal plants. International Journal of Food Properties 2013; 16; 1117-1126. https://doi.org/10.1080/10942912.2011.576360
Oyaizu M. Studies on products of the browning reaction prepared from glucose amine. Japanese Journal of Nutrition 1986; 44: 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E1. Analytical Biochemistry 1999; 269: 337-341. https://doi.org/10.1006/abio.1999.4019
Oyaizu M. Studies on products of the browning reaction prepared from glucose amine. Jpn J Nutr 1986; 44: 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
Celiktas OY, Hames Kocabas EE, Bedir E, Verdar Sucan O, Baser KHC, Antimicrobial activities of methanolic extract andessential oils of Rosmarinus officinalis, depending on location and seasonal variations. Food Chem 2007; 100: 553-559. https://doi.org/10.1016/j.foodchem.2005.10.011
Arras G, Usai M. Fungitoxic activity of 12 essential oils against four postharvest Citrus pathogens: chemical analysis of Thymus capitatus oil and its effect in subatmospheric pressure conditions. J Food Prot 2001; 64: 1025-1029. https://doi.org/10.4315/0362-028X-64.7.1025
Baser KHC, Kirimer N, Tumen G, Duman H. Composition of the essential oils of Thymus canaoviridis Jalas. J Essent Oil Res 1998; 10: 199-200. https://doi.org/10.1080/10412905.1998.9700879
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