Abstract
The prevalence of H9N2 influenza virus in dogs was first time observed in Fars province of Iran. A total of 182 dogs were selected from the clinical cases at the Small Animal Clinic of Veterinary Medicine School, Shiraz University. After obtaining history, physical examination was performed and blood samples were obtained for serological examination (Eliza and HI assay) for the detection of H9N2-specific antibodies. Associated factors (age, breed, diet, place, presence of other dogs, general symptoms, respiratory and gastrointestinal signs) were also evaluated. The positive results showed that 81.7 % of ELISA positive cases had titer ? 32 for H9N2 influenza in HI test. Although positive result were found more in dogs with general or respiratory signs, no significant differences were observed in the evaluated factors and seropositivity. This research showed high seroprevalence of Ab against H9N2 in dogs and made this hypothesis that H9N2 may be important in dogs in virus persistence. Additional research is needed for detection of epidemiologic role of dogs in transmission and pathogenesis of H9N2 in dogs and humans.
References
Wright PF, Webster RG. In Orthomyxoviruses. Lippincott William & Wilkins, Philadelphia, 2001; pp. 1533-1539.
Fouchier RA, Munster V, Wallensten A, et al. Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black headed gulls. J Virol 2005; 79: 2814-22. http://dx.doi.org/10.1128/JVI.79.5.2814-2822.2005
Kianizadeh M, Pourbakhsh SA, Toroghi R, Momayez R. Pathogenicity and haemagglutinin gene sequence analysis of Iranian avian influenza H9N2 viruses isolated during (1998-2001). Iranian J Vet Res 2006; 7(3): 37-40.
Hadipour MM. H9N2 Avian Influenza Virus Antibody Titers in Human Population in Fars Province, Iran. Brazilian J Poult Sci 2010; 12(3): 161-64.
Hinshaw VS, Webster RG, Easterday BC, Bean Jr. Replication of avian influenza A viruses in mammals. Infect Immun 1981; 34: 354-61.
Song D, Kang B, Lee C, et al. Transmission of avian influenza virus (H3N2) to dogs. Emerg Infect Dis 2008; 14: 741-46. http://dx.doi.org/10.3201/eid1405.071471
Crawford PC, Dubovi EJ, Castleman WL, Stephenson I, Gibbs EPJ, Chen L, Smith C, Hill RC, Ferro P, Pompey J, Bright RA, Medina MJ. Influenza Genomics Group. Johnson CM, Olsen CW, Cox NJ, Klimov AI, Katz JM, Donis RO. Transmission of equine influenza virus to dogs. Science 2005; 310: 482-85. http://dx.doi.org/10.1126/science.1117950
Guan Y, Poon LL, Cheung CY, et al. H5N1 influenza: a protean pandemic threat. Proc Natl Acad Sci USA 2004; 101: 8156-61. http://dx.doi.org/10.1073/pnas.0402443101
Thaweesak S, Alongkorn A, Rungroj J, et al. Fatal avian influenza A H5N1 in a dog. Emerg Infect Dis 2006; 12: 1744-47. http://dx.doi.org/10.3201/eid1211.060542
Lee C, Song D, Kang B, et al. A serological survey of avian origin canine H3N2 influenza virus in dogs in Korea. Vet Microbiol 2009; 137: 359-62. http://dx.doi.org/10.1016/j.vetmic.2009.01.019
Song D, Lee C, Kang B, et al. Experimental infection of dogs with avian-origin canine influenza A virus (H3N2). Emerg Infect Dis 2009; 15: 56-58. http://dx.doi.org/10.3201/eid1501.080755
Amirsalehy H, Nili H, Mohammadi A. Experimental infection of dogs with avian influenza (H9N2) virus. 3rd International Oxford Influenza Conference Influenza. Zoonotic influenza & human health, Oxford, England, 2010; 21-23.
Li C, Yu K, Tian G, et al. Evolution of H9N2 influenza viruses from domestic poultry in Mainland China. Virol 2005; 340: 70-83. http://dx.doi.org/10.1016/j.virol.2005.06.025
Xu C, Fan W, Wei R, Zhao H. Isolation and identification of swine influenza recombinant A/Swine/Shandong/1/2003(H9N2) virus. Microbes Infect 2004; 6: 919-25. http://dx.doi.org/10.1016/j.micinf.2004.04.015
Xu KM, Li KS, Smith GJ, et al. Evolution and molecular epidemiology of H9N2 influenza A viruses from quail in southern China, 2000 to 2005. J Virol 2007; 81: 2635-45. http://dx.doi.org/10.1128/JVI.02316-06
Alexander DJ. A review of avian influenza in different bird species. Vet Microbiol 2000; 74: 3-13. http://dx.doi.org/10.1016/S0378-1135(00)00160-7
Perk S, Panshin A, Shihmanter E, et al. Ecology and molecular epidemiology of H9N2 avian influenza viruses isolated in Israel during 2000-2004 epizootic. Dev Biol (Basel) 2006; 124: 201-209.
Abbaszadeh M, Nazifi S, Mohsenifard E, Ansari-Lari M. Serologic prevalence of antibodies against avian origin influenza virus in dogs referred to the Veterinary Clinic at Shiraz University. Comp Clin Pathol 2011; DOI 10.1007.
Holt D, Moyer M, Brown DC. Serologic prevalence of antibodies against canine influenza virus (H3N8) in dogs in a metropolitan animal shelter. JAVMA 2010; 237: 71-73. http://dx.doi.org/10.2460/javma.237.1.71
Guo YJ, Krauss S, Senne DA, et al. Characterization of the pathogenecity of members of the newly established H9N2 influenza virus lineages in Asia. Virol 2000; 267: 279-88. http://dx.doi.org/10.1006/viro.1999.0115
Kruth SA, Carman S, Weese JS. Seroprevalence of antibodies to canine influenza virus in dogs in Ontario. Can Vet J 2008; 49: 800-802.
Beeler E. Influenza in dogs and cats. Vet Clin North Am Small Anim Pract 2009; 39: 251-64. http://dx.doi.org/10.1016/j.cvsm.2008.10.011
Lipatov AS, Govorkova EA, Webby RJ. Influenza: emergence and control. J Virol 2004; 78: 8951-96. http://dx.doi.org/10.1128/JVI.78.17.8951-8959.2004
Mosleh N, Dadras H, Mohammadi A. Evaluation of H9N2 avian influenza virus dissemination in various organs of experimentally infected broiler chickens using RT-PCR. Iranian J Vet Res 2009; 10(1): 12-19.
Crawford PC. Canine influenza: Frequently asked questions from dog owners. University of Florida. 2009; Available at: http://www.vetmed.ufl.edu/college/pr/documents/CanineinfluenzaFAQDogowners_001.pdf