Keywords
How to Cite
Abstract
We here investigated the inhibitory effect of Brazilian propolis on intracellular lipid accumulation. Seven ethanolic extracts of Brazilian propolis were tested for their inhibitory effect on intracellular lipid accumulation in 3T3-L1 cells. Among the seven Brazilian propolis, the extracts AF-08 and AF-18 had inhibitory effects on intracellular lipid accumulation. In particular, the Brazilian propolis AF-08 exhibited a potential inhibitory effect on intracellular lipid accumulation.Thus, these results suggest that the Brazilian propolis AF-08 contributes to the prevention of metabolic syndrome. In addition, five known triterpenoids were isolated from the Brazilian propolis AF-08: betulonic acid, moronic acid, 3-oxo oleanolic acid, masticadienonic acid, and anwnweizonic acid. Four of the triterpenoids exhibited an inhibitory effect on intracellular lipid accumulation. Thus, these triterpenoids may be important as antiobesity agents that inhibit the metabolic syndrome.
References
Ghisalberti EL. Propolis: a review. Bee World 1979; 60: 59- 84.
Marcucci MC. Propolis: Chemical composition, biological properties and therapeutic activity. Apidologie 1995; 26: 83- 96. http://dx.doi.org/10.1051/apido:19950202
Kumazawa S, Yoneda M, Shibata I, et al. Direct evidence for the plant origin of Brazilian propolis by the observation of honeybee behavior and phytochemical analysis. Chem Pharm Bull 2003; 51 (6): 740-742. http://dx.doi.org/10.1248/cpb.51.740
Scazzocchio F, D’Auria FD, Alessandrini D, et al. Multifactorial aspects of antimicrobial activity of propolis. Microbiol Res 2006; 161: 327-333. http://dx.doi.org/10.1016/j.micres.2005.12.003
Shimizu T, Hino A, Tsutsumi A, et al. Anti-influenza virus activity of propolis in vitro and its efficacy against influenza infection in mice. Antivir Chem Chemother 2008; 19 (1): 7-13.
Tan-no K, Nakajima T, Shoji T, et al. Anti-inflammatory effect of propolis through inhibition of nitric oxide production on carrageenin-induced mouse paw edema. Biol Pharm Bull 2006; 29 (1): 96-99. http://dx.doi.org/10.1248/bpb.29.96
Murad JM, Calvi SA, Soares AMVC, et al. Effects of propolis from Brazil and Bulgaria on fungicidal activity of macrophages against Paracoccidioides brasiliensis. J Ethnopharmacol 2002; 79: 331-334. http://dx.doi.org/10.1016/S0378-8741(01)00404-4
Shimizu K, Ashida H, Matsuura Y, et al. Antioxidant bioavailability of artepillin-C in Brazilian propolis. Arch Biochem Biophys 2004; 424: 181-188. http://dx.doi.org/10.1016/j.abb.2004.02.021
Nakajima Y, Shimazawa M, Mishima S, et al. Water extract of propolis and its main constituents, caffeoylquinic acid derivatives, exert neuroprotective effects via antioxidant actions. Life Sci 2007; 80: 370-377. http://dx.doi.org/10.1016/j.lfs.2006.09.017
Izuta H, Narahara Y, Shimazawa M, et al. 1,1-Diphenyl-2- picrylhydrazyl radical scavenging activity of bee products and their constituents determined by ESR. Biol Pharm Bull 2009; 32 (12): 1947-1951. http://dx.doi.org/10.1248/bpb.32.1947
Kopelman PG. Obesity as a medical problem. Nature 2000; 404: 635-643.
Mann CC. Public health: provocative study says obesity may reduce U.S. life expectancy. Science 2005; 307: 1716-1717. http://dx.doi.org/10.1126/science.307.5716.1716
Ito M, Ito J, Kitazawa H, et al. (-)-Ternatin inhibits adipogenesis and lipid metabolism in 3T3-L1 cells. Peptides 2009; 30: 1074-1081. http://dx.doi.org/10.1016/j.peptides.2009.02.008
Furuyashiki T, Nagayasu H, Aoki Y, et al. Tea catechin suppresses adipocyte differentiation accompanied by downregulation of PPAR?2 and C/EBP? in 3T3-L1 cells. Biosci Biotechnol Biochem 2004; 68: 2353-2359. http://dx.doi.org/10.1271/bbb.68.2353
Ahn J, Lee H, Kim S, Park J, Ha T. The anti-obesity effect of quercetin is mediated by the AMPK and MAPK signaling pathways. Biochem Biophys Res Commun 2008; 373: 597- 608. http://dx.doi.org/10.1016/j.bbrc.2008.06.077
Wada T, Ihunnah CA, Gao J, et al. Estrogen sulfotransferase inhibits adipocyte differentiation. Mol Endocrinol 2011; 25: 1612-1623. http://dx.doi.org/10.1210/me.2011-1089
Yahagi T, Daikonya A, Kitanaka S. Flavonol acylglycosides from flower of Albizia julibrissin and their inhibitory effects on lipid accumulation in 3T3-L1 cells. Chem Pharm Bull 2012; 60: 129-136. http://dx.doi.org/10.1248/cpb.60.129
Ito J, Chang FR, Wang HK, et al. Anti-AIDS agents. 48.Anti- HIV activity of moronic acid derivatives and the new melliferone-related triterpenoid isolated from Brazilian propolis. J Nat Prod 2001; 64: 1278-1281. http://dx.doi.org/10.1021/np010211x
Zhang P, Hao J, Liu J, et al. Efficient synthesis of morolic acid and related triterpenes starting from betulin. Tetrahedron 2009; 65: 4304-4309. http://dx.doi.org/10.1016/j.tet.2009.03.100
Konoike T, Takahashi K, Araki Y, et al. Practical partial synthesis of myriceric acid A, an endothelin receptor antagonist, from oleanolic acid. J Org Chem 1997; 62: 960- 966. http://dx.doi.org/10.1021/jo9615864
Mulholland D A, Nair JJ. Triterpenoids from dysoxylum pettigrewianum. Phytochemistry 1994; 37 (5): 1409-1411. http://dx.doi.org/10.1016/S0031-9422(00)90421-7
Huang C, Zhang Y, Gong Z, et al. Berberine inhibits 3T3-L1 adipocyte differentiation through the PPAR? pathway. Biochem Biophys Res Commun 2006; 348: 571-578. http://dx.doi.org/10.1016/j.bbrc.2006.07.095
Hata K, Hiwatashi K, Itoh M, et al. Inhibitory effects of lupeol on 3T3-L1 preadipocyte differentiation. Phytochem Lett 2008; 1: 191-194. http://dx.doi.org/10.1016/j.phytol.2008.09.007
Ahn EK, Oh JS. Lupenone isolated from adenophora triphylla var. japonica extract inhibits adipogenic differentiation through the downregulation of PPAR? in 3T3-L1 cells. Phytother Res 2013; 27: 761-766. http://dx.doi.org/10.1002/ptr.4779
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright (c) 2014 Yasuhito Nobushi, Naoki Oikawa, Yuzo Okazaki, Shigetoshi Tsutsumi, Yong Kun Park, Masahiko Kurokawa , Ken Yasukawa