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The in vitro cytotoxic activity of hexane, ethyl acetate and butanol extracts of the sea-cucumber Isostichopus badionotus (Holothuroidea) was tested against normal cells (Vero), human cervical carcinoma (HeLa) and breast adenocarcinoma (MCF-7 and MDA-MB-231) ATCC cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Hexane extracts from body walls and viscera showed high cytotoxic activity against HeLa cells (IC50’s = 48.5 and 42.5 µg mL-1, respectively), while the ethyl acetate extract of body walls was considered low active (IC50 = 98.3 μg mL-1). In addition, the body walls hexane extract showed a good selectivity index value of 12.0.
References
Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Maxwell D. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer 2007; 127: 2893-2917. http://dx.doi.org/10.1002/ijc.25516
Nussbaumer S, Bonnabry P, Veuthey JL, Fleury-Souverain S. Analysis of anticancer drugs: A review. Talanta 2011; 85: 2265-2289. http://dx.doi.org/10.1016/j.talanta.2011.08.034
Rebucci M, Michiels C. Molecular aspects of cancer cell resistance to chemotherapy. Biochem Pharmacol 2013; 85: 1219-1226. http://dx.doi.org/10.1016/j.bcp.2013.02.017
Blunden G. Biologically active compounds from marine organisms. Phytother Res 2001; 15: 89-94. http://dx.doi.org/10.1002/ptr.982
Blunt JW, Copp BR, Munro MHG, Northcote PT, Prinsep MR. Marine natural products. Nat Prod Rep 2004; 31: 160-258. http://dx.doi.org/10.1039/c3np70117d
Simmons TL, Andrianasolo E, McPhail K, Flatt P., Gerwick WH. Marine natural products as anticancer drugs. Mol Cancer Ther 2005; 4: 333-342.
Conand C.,Byrne M. A review of recent developments in the world sea cucumber fisheries. Marine Fisheries Review 1993; 55: 1-15.
Chen J. Overview of sea cucumber farming and sea ranching practices in China. SPC Beche-de-mer Information Bulletin 2003; 18: 18-23.
Chen J. Present status and prospects of sea cucumber industry in China. In: Conand C, Purcell S, Uthicke S, Hamel JF, Mercier A, editors. Advances in sea cucumber aquaculture and management. FAO Fisheries Technical Paper No. 463 Rome: FAO 2004; p. 25-38.
Park SY, Lim HK, Lee S, Hwang HC, Cho SK, Cho M. Pepsin-solubilized collagen (PSC) from red sea cucumber (Stichopus japonicus) regulates cell cycle and the fibronectin synthesis in HaCat cell migration. Food Chem 2012; 132: 487-492. http://dx.doi.org/10.1016/j.foodchem.2011.11.032
Wen W, Chaoqun H, Zhang L, Luo P, Zhao Z, Fan S, Su T. The application of PCR-RFLP and FINS for species identification used in sea cucumbers (Aspidochirotida: Stichopodidae) products from the market. Food Control 2010; 21: 403-407. http://dx.doi.org/10.1016/j.foodcont.2009.06.014
Aydin M, Sevgili H, Tufan B, Emre Y, Köse S. Proximate composition and fatty acid profile of three different fresh and dried commercial sea cucumbers from Turkey. International Journal of Food Science and Technology 2011; 46: 500-508. http://dx.doi.org/10.1111/j.1365-2621.2010.02512.x
Bordbar S, Anwar F, Saari N. High-Value components and bioactives from sea cucumbers for functional foods- A review. Mar Drugs 2011; 9: 1761-1805. http://dx.doi.org/10.3390/md9101761
Guzmán HM, Guevara CA. Population structure, distribution and abundance of three commercial species of sea cucumber (Echinodermata) in Panama. Caribb. J. Sci. 2002; 38: 230-238.
Purcell, SW, Samyn Y, Conand C. Commercially important sea cucumbers of the world. FAO Species Catalogue for Fishery Purposes No. 6. Rome: FAO; 2012.
Pérez-Vega JA, Olivera-Castillo L, Gómez-Ruiz JA, Hernández-Ledesma B. Release of multifunctional peptides by gastrointestinal digestion of sea cucumber (Isostichopus badionotus). J Funct Foods 2013; 5: 869-877. http://dx.doi.org/10.1016/j.jff.2013.01.036
Schlie-Guzmán M, García-Carrancá A, González-Esquinca A. In Vitro and In Vivo Antiproliferative Activity of Laherradurin and Cherimolin-2 of Annona diversifolia Saff. Phytother Res 2009; 23: 1128–1133. http://dx.doi.org/10.1002/ptr.2760
Manosroi J, Dhumtanom P, Manosroi A. Anti-proliferative activity of essential oil extracted from Thai medicinal plants on KB and P388 cell lines. Cancer Lett 2006: 235: 114–120. http://dx.doi.org/10.1016/j.canlet.2005.04.021
Vonthron-Sénécheau C, Weniger B, Ouattara M, et al. In vitro antiplasmodial activity and cytotoxicity of ethnobotanically selected Ivorian plants. J Ethnopharmacol 2003, 87: 221-225. http://dx.doi.org/10.1016/S0378-8741(03)00144-2
Xu J, Guo S, Du L, Wang YM, Sugawara T, Hirata T, Xue CH. Isolation of cytotoxic glucoerebrosides and longchain bases from sea cucumber Cucumaria frondosa using high speed counter-current chromatography. J Oleo Sci 2013, 62: 133-142. http://dx.doi.org/10.5650/jos.62.133
Kalinin V, Aminin DL, Avilov SA, Silchenko AS, Stonik VA. Triterpene glycosides from sea cucumbers (Holothurioidea, Echinodermata). Biological activities and functions. In: Atta-ur-Rahman editor. Studies in Natural Products Chemistry. Elsevier B.V. 2008; p. 135-196.
Elyakov GB, Kuznetsova TA, Conde C, Kalinovskaya NI, Kalinovskii AI, Smetanina OF. Glycosides of marine invertebrates VIII. Steroid glycosides of the holothurian Isostichopus badionotus. Chemistry of Natural Compounds 1979, 6: 799-708.
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Copyright (c) 2014 Aida R. Pérez-Espadas, María J. Verde-Star, Catalina Rivas-Morales, Azucena Oranday-Cárdenas, María E. Morales-Rubio, Lorena V. León-Deniz, Jaqueline Canul-Canché , Leovigildo Quijano