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Abstract
Zizyphus nummularia (Burm. F.) (Family: Rhamnaceae) used as a folk medicine. The study was designed to lay down the various physicochemical and phytochemical standards which will be helpful to ensure the purity, safety, and efficacy of this medicinal plant. In the lime light of fewer and less potent antioxidant extracts, it is screened for antioxidant potency also. All physico-chemical properties were found within the measurable amount. Preliminary phytochemical analysis confirmed the presence of alkaloids, flavonoids, tannins and steroids. Total phenolic content and flavonoid content was found to be 10.7±0.1 and 2.57±0.1 respectively. Extract also found to posses significant antioxidant activity which can be correlated with phenolic content. IC50 of 0.042 mg/mL was found during DPPH radical scavenging activity. 2.75 mmol AA/ mg extract was found to be as total antioxidant capacity along with a concentration of 383.4 µg/ml during reducing power activity. The study would help in the authentication stem bark useful for laying down pharmacopoeial standards and help in finding improved antioxidant agents from the extract. It is found to be posse’s significant antioxidant property, possibly by adsorbing and neutralizing free radicals.
References
Calixto JB. Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents). Braz J Med Biol Res 2000; 33: 179-89. https://doi.org/10.1590/S0100-879X2000000200004
Mukharjee PK. GMP for Botanicals, Regulatory and Quality Issues on Phytomedicines. 1st ed. New Delhi: Business Horizons; 2003; p. 219.
Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal Plants. New Delhi: CSIR; 1956; p. 261.
Kirtikar KR, Basu BD. Indian Medicinal Plants. Vol. I Dehradun, India: BSMP Publishers; 1975; p. 592.
CAZRI. Bordi-Ziziphus nummularia, A Shrub of Indian Arid Zone. (Eds. H.S. Mann & S.K. Saxena). CAZRI Monograph. 1981; p. 13.
Anonymous, The Wealth of India (Raw material) Vol XI: X-Z. Council of Industrial and Scientific Research, New Delhi; 1989; p. 111-124.
Shah AH, Khan RMA, Maurya SK, Singh VP. Nummularine-S: A cyclopeptide alkaloid from the stem bark of Zizyphus nummularia. Phytochem 1989; 28(1): 305-7. https://doi.org/10.1016/0031-9422(89)85072-1
Kauffmann TR. The Alkaloids Chemistry & Physiology, Monske. RHF edition, Academic Press, London; 1975; Vol. 15: p. 165.
Anonymous. Quality Control Methods for Medicinal Plant Materials. Geneva: Office of the Publications, World Health Organization; 1998; 8-46.
Kumar D, Gupta J, Kumar S, Arya R, Kumar T, Gupta A. Pharmacognostic evaluation of Cayratia trifolia (Linn.) leaf. Asian Pac J Trop Biomed 2012; 2: 6-10. https://doi.org/10.1016/S2221-1691(11)60180-9
Katara A, Kumar PC, Tyagi AK, Singh P. Phytochemical investigation and antimicrobial activity of Leucas cephalotus Roth. Spreng whole herb. Pharm Lett 2010; 2: 284-96.
Khandelwal KR. Practical Pharmacognosy techniques and experiments. New Delhi: Nirali Prakashan; 2002; 15-163.
Kokate CK. Practical Pharmacognosy. 1st ed. New Delhi: Vallabh Prakashan; 2005; p. 111.
Kaur C, Kapoor HC. Anti-oxidant activity and total phenolic content of some Asian vegetables. Int J Food Sci Tech 2002; 37: 153-61. https://doi.org/10.1046/j.1365-2621.2002.00552.x
Panday MM, Katara A, Pandey G, Rastogi S, Rawat AKS. An important traditional indian drug of Ayurveda Jatamansi and its substitute Bhootkeshi: Chemical profiling and antioxidant activity. Evid Based Complement Alternat Med 2013; Article ID 142517. https://doi.org/10.1155/2013/142517
Quettier DC, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx MC, et al. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J Ethnopharmacol 2000; 72: 35-42. https://doi.org/10.1016/S0378-8741(00)00196-3
Hirano R, Sasamoto W, Matsumoto A, Itakura H, Igarashi O, Kondo K. Antioxidant ability of various flavonoids against DPPH radicals and LDL oxidation. J Nutr Sci Vitaminol 2001; 47(5): 357-62. https://doi.org/10.3177/jnsv.47.357
Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Japanese J Nut 1986; 44: 307-15. https://doi.org/10.5264/eiyogakuzashi.44.307
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem 1999; 269: 337-41. https://doi.org/10.1006/abio.1999.4019
Liu KC, Yang SL, Roberts MF, Elford BC, Phillipson JD. Antimalarial activity of Artemisia annua flavonoids from whole plants and cell cultures. Plant Cell Rep 1992; 11: 637-40. https://doi.org/10.1007/BF00236389
Jayanthi P, Lalitha P. Reducing power of the solvent extracts of Eichhornia crassipes (Mart.) Solms. Int J Pharm Pharm Sci 2011; 3: 126-8.
Zhao Z, Liang Z, Guo P. Macroscopic identification of Chinese medicinal materials: Traditional experiences and modern understanding. J Ethnopharmacol 2011; 131: 556-1. https://doi.org/10.1016/j.jep.2011.01.018
Sutar RC, Kakaichelvan VK. Evaluation of antioxidant activity of leaf extracts of Holoptelea integrifolia (Roxb.) planch. Int J App Pharm 2014; 6: 6-8.
Carbanaro M, Mattera M, Nicoli S, Bergamo P, Cappelloni M. Modulattion of antioxidant compounds in organic vs conventional fruit (peach, Prunus persica and pear, Pyrus communis L.). J Agric Food Chem 2002; 50: 5458-62. https://doi.org/10.1021/jf0202584
Yildirim A, Mavi A, Oktay M, Kara AA, Algur OF, Bilaloglu Vol. 15. Comparison of antioxidant and antimicrobial activities of Tilia (Tilia argentea Desf ex DC), sage (Salvia triloba L.), and Black tea (Camellia sinensis) extracts. J Agri Food Chem 2000; 48: 5030-4. https://doi.org/10.1021/jf000590k
Ferreira ICFR, Baptista P, Vilas-Boas M, Barros L. Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: individual cap and stipe activity. Food Chem 2007; 100: 1511-6. https://doi.org/10.1016/j.foodchem.2005.11.043
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