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Abstract
Germander (Teucrium, Lamiaceae) is a genus commonly used as a medicinal herb in the middle east. The aim of this study was to estimate total polyphenols and antioxidant activity of different extracts from two Syrian Teucrium species (Teucrium polium and Teucrium creticum). Total polyphenols content was measured using Folin-Ciocalteu assay and the antioxidant activity was evaluated in vitro by DPPH assay (free radical scavenging activity against 1, 1 –diphenyl-2-picrylhydrazyl). The total polyphenols content and antioxidant activity of T. creticum extracts were higher than T. polium ones. Phenols contents ranged from 16.388 to 105.672 mg GA E/ g dry weight extract. The extracts showed significant scavenging activity against DPPH, with IC50 values ranging between 0.195 and 0.710 mg/ml. The methanolic extracts show higher antioxidant activity from the two species. A good correlation between DPPH scavenging activities and contents of polyphenols was found. These results show that Syrian Teucrium species, especially T. creticum least Known among all, is a rich source of phenols and natural antioxidant compounds, that can be used as a natural food preservative.
References
Van Acker SAB, Van Balen GP, Van den Berg D, Bast A, Van der Vijgh WJ. Influence of iron chelation on the antioxidant activity of flavonoids. Biochem Pharmacol 1998; 56(8): 935-943. https://doi.org/10.1016/S0006-2952(98)00102-6
Aruoma OI. Nutrition and health aspects of free radicals and antioxidants. Food Chem Toxicol 1994; 32(7): 671-683. https://doi.org/10.1016/0278-6915(94)90011-6
Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med 1996; 20(7): 933-956. https://doi.org/10.1016/0891-5849(95)02227-9
Ruiters AK, Tilney PM, Van Vuuren SF, Viljoen AM, Kamatou GPP, Van Wyk B-E. The anatomy, ethnobotany, antimicrobial activity and essential oil composition of southern African species of Teucrium (Lamiaceae). S Afr J Bot 2016; 102: 175-185. https://doi.org/10.1016/j.sajb.2015.06.008
Rahmouni F, Hamdaoui L, Rebai T. In vivo anti-inflammatory activity of aqueous extract of Teucrium polium against carrageenan-induced inflammation in experimental models. Arch Physiol Biochem 2017; 123(5): 313-321. https://doi.org/10.1080/13813455.2017.1333517
Darabpour E, Motamedi H, Nejad SMS. Antimicrobial properties of Teucrium polium against some clinical pathogens. Asian Pac J Trop Med 2010; 3(2): 124-127. https://doi.org/10.1016/S1995-7645(10)60050-8
Twaij HAA, Albadr AA, Abul-Khail A. Anti-Ulcer Activity of Teucrium polium. Int J Crude Drug Res 1987; 25(2): 125-128.
Abdollahi M, Karimpour H, Monsef-Esfehani HR. Antinociceptive effects of Teucrium polium L. total extract and essential oil in mouse writhing test. Pharmacol Res 2003; 48(1): 31-35. https://doi.org/10.1016/S1043-6618(03)00059-8
Autore G, Capasso F, De Fusco R, Fasulo MP, Lembo M, Mascolo N, et al. Antipyretic and antibacterial actions of Teucrium polium (L.). Pharmacol Res Commun 1984; 16(1): 21-29. https://doi.org/10.1016/S0031-6989(84)80101-0
Rasekh H, Khoshnood-Mansourkhani M, Kamalinejad M. Hypolipidemic effects of Teucrium polium in rats. Fitoterapia 2001; 72(8): 937-939. https://doi.org/10.1016/S0367-326X(01)00348-3
Gharaibeh MN, Elayan HH, Salhab AS. Hypoglycemic effects of Teucrium polium. J Ethnopharmacol 1988; 24(1): 93-99. https://doi.org/10.1016/0378-8741(88)90139-0
Suleiman MS, Abdul-Ghani AS, Al-Khalil S, Amin R. Effect of Teucrium polium boiled leaf extract on intestinal motility and blood pressure. J Ethnopharmacol 1988; 22(1): 111-116. https://doi.org/10.1016/0378-8741(88)90236-X
Ljubuncic P, Dakwar S, Portnaya I, Cogan U, Azaizeh H, Bomzon A. Aqueous Extracts of Teucrium polium Possess Remarkable Antioxidant Activity In vitro. Evid-Based Complementary Altern Med 2006; 3(3): 329-338. https://doi.org/10.1093/ecam/nel028
Ötles S. Methods of Analysis of Food Components and Additives. Chemical & Functional Properties of Food Components. 3rd ed. Boka Raton: CRC Press 2005. https://doi.org/10.1201/9780203503751
Takao T, Kitatani F, Watanabe N, Yagi A, Sakata K. A. Simple Screening Method for Antioxidants and Isolation of Several Antioxidants Produced by Marine Bacteria from Fish and Shellfish. Biosci Biotechnol Biochem 1994; 58(10): 1780-1783. https://doi.org/10.1271/bbb.58.1780
Kumarasamy Y, Byres M, Cox PJ, Jaspars M, Nahar L, Sarker SD. Screening seeds of some Scottish plants for free radical scavenging activity. Phytother Res 2007; 21(7): 615-621. https://doi.org/10.1002/ptr.2129
Stalikas CD. Extraction, separation, and detection methods for phenolic acids and flavonoids. J Sep Sci 2007; 30(18): 3268-3295. https://doi.org/10.1002/jssc.200700261
El Atki Y, Aouam I, E Kamari F, Taroq A, Lyoussi B and Abdellaoui A. Antioxidnt activity of two wild Teucrium species from Morocco. Int J Pharm Sci & Res 2019; 10(6): 2723-2729. https://doi.org/10.13040/IJPSR.0975-8232.10(6).2723-29
Stankovic MS. Niciforovic N. Mihailovie V, Topuzovic M and Solujic S. Antioxidant activity, total phenolic content and flavonoids concentrations of different plant parts of Teucrium polium L. subsp. Polium Acta Soc Bot Pol 2012; 81(2): 117-122. https://doi.org/10.5586/asbp.2012.010
Marinova EM, Yanishlieva NV. Antioxidative activity of extracts from selected species of the family Lamiaceae in sunflower oil. Food Chem 1997; 58(3): 245-248. https://doi.org/10.1016/S0308-8146(96)00223-3
Abdillah S, Tambunan RM, Farida Y, Sandhiutami NMD, Dewi RM. Phytochemical screening and antimalarial activity of some plants traditionally used in Indonesia. Asian Pac J Trop Dis 2015; 5(6): 454-457. https://doi.org/10.1016/S2222-1808(15)60814-3
Martelli G, Giacomini D. Antibacterial and antioxidant activities for natural and synthetic dual-active compounds. Eur J Med Chem 2018; 158: 91-105. https://doi.org/10.1016/j.ejmech.2018.09.009
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