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Abstract
In this paper, the polymeric micelle was fabricated with amphiphilic block copolymer which was synthesized by the condensation of mechanochemically produced PBzMA macromonomer with a-methyl-w-aminopropoxy polyoxyethylene. Although the polymeric micelles containing 5-fluorouracil (5-FU) or rohdamine showed the narrow size distribution as well as blank polymeric micelles, the particle diameter of the polymeric micelles containing 5-FU or rohdamine was larger than that of blank polymeric micelle. Polymeric micelle containing 5-FU slowly released 5-FU and about 10 % of 5-FU was released within 72 h. The present polymeric micelle was uptaken by A549 cells, but HepG2 cells did not. Cytotoxicity of Polymeric micelle containing 5-FU was not observed against HepG2 and A549 cells in this experimental condition. It was considered that the slow release of 5-FU from Polymeric micelle might cause the insufficient cytotoxicity.
References
Yokoyama M, Miyauchi M, Yamada N, Okano T, Kataoka K, Inoue S. Polymer micelles as novel drug carrier: adriamycin-conjugated poly(ethylene glycol)-poly(aspartic acid) block copolymer. J Control Rel 1990; 11: 269-278. http://dx.doi.org/10.1016/0168-3659(90)90139-K
Kohori F, Sakai K, Aoyagi T, Yokoyama M, Sakurai Y, Okano T. Preparation and characterization of thermally responsive block copolymer micelles comprising poly (N-isopropylacrylamide-b-DL-lactide). J Control Rel 1998; 55: 87-98. http://dx.doi.org/10.1016/S0168-3659(98)00023-6
Yasugi K, Nagasaki Y, Kato M, Kataoka K. Preparation and characterization of polymer micelles from poly(ethylene glycol)-poly(D,L-lactide) block copolymers as potential drug carrier. J Control Rel 1999; 62: 89-100. http://dx.doi.org/10.1016/S0168-3659(99)00028-0
Akiyoshi K, Kang E-C, Kurumada S, Sunamoto J, Principi T, Winnik FM. Controlled association of amphiphilic polymers in water: thermosensitive nanoparticles formed by self-assembly of hydrophobically modified pullulans and poly(N-isopropylacrylamides). Macromolecules 2000; 33: 3244-3249. http://dx.doi.org/10.1021/ma991798d
Katayama Y, Sonoda T, Maeda M. A polymer micelle responding to the protein kinase a signal. Macromolecules 2001; 34: 8569-8573. http://dx.doi.org/10.1021/ma010966a
Kataoka K, Harada A, Nagasaki Y. Block copolymer micelles for drug delivery: design, characterization and biological significance. Adv Drug Deliv Rev 2001; 47: 113-131 and references cited therein.
Savic R, Luo L, Eisenberg A, Maysinger D. Micellar nanocontainers distribute to defined cytoplasmic organelles. Science 2003; 300: 615-618. http://dx.doi.org/10.1126/science.1078192
Francis MF, Cristea M, Winnik FM. Polymeric micelles for oral drug delivery : Why and how. Pure Appl Chem 2004; 76: 1321-1335, and references cited therein.
Djordjevic J, Barch M, Uhrich KE. Polymeric micelles based on amphiphilic scorpion-like macromolecules: novel carriers for water-insoluble drugs. Pharm Res 2005; 22: 24-32. http://dx.doi.org/10.1007/s11095-004-9005-3
Bae Y, Nishiyama N, Fukushima S, Koyama H, Yasuhiro M, Kataoka K. Preparation and biological characterization of polymeric micelle drug carriers with intracellular pH-triggered drug release property: tumor permeability, controlled subcellular drug distribution, and enhanced in vivo antitumor efficacy. Bioconjugate Chem 2005; 16: 122-130. http://dx.doi.org/10.1021/bc0498166
Ito K. Polymeric design by macromonomer technique. Prog Polym Sci 1998; 23: 581-620. http://dx.doi.org/10.1016/S0079-6700(97)00049-X
Ito K, Kawaguchi S. Poly(macromonomers): homo-and copolymerization . Adv Polym Sci 1999; 142: 129-178. http://dx.doi.org/10.1007/3-540-68310-0_3
Maniruzzaman M, Kawaguchi S, Ito K. Micellar copolymerization of styrene with poly(ethylene oxide) macromonomer in water: approach to unimolecular nanoparticles via pseudo-liming radical polymerization. Macromolecules 2000; 33: 1583-1592. http://dx.doi.org/10.1021/ma991272t
Kondo S, Tsukamoto M, Sasai Y, Yamauchi Y, Kuzuya M. Novel synthesis of macromonomers by mechanochemical reaction for the application to polymeric micelles. J Phar Nutrit Sci 2011; 1: 124-129. http://dx.doi.org/10.6000/1927-5951.2011.01.02.06
Kondo S, Omoto M, Sawama Y, Sasai Y, Tatematsu K, Yamauchi Y, Kuzuya M. Synthesis of amphiphilic blockcopolymer using mechanically produced macromonomers possessing anhydrate as a terminal group and its application to polymeric micelles. J Phar Nutrit Sci 2014; 4: 37-42. http://dx.doi.org/10.6000/1927-5951.2014.04.01.6
Iijima M, Nagasaki Y, Okada T, Kato M, Kataoka K. Core-Polymerized Reactive Micelles from Heterotelechelic Amphiphilic Block Copolymers. Macromolecules 1999; 32: 1140-1146. http://dx.doi.org/10.1021/ma9815962
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Copyright (c) 2016 Shin-ichi Kondo, Sayaka Kimura, Kenjiro Tatematsu, Yuka Sawama, Naoki Doi, Yasushi Sasai, Yukinori Yamauchi, Masayuki Kuzuya